Journal: iScience

Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment

doi: 10.1016/j.isci.2026.117739

Figure Lengend Snippet: IL-4 and Arg remodel macrophages and promote their migration (A) Schematic illustration of the regulatory role of IL-4 on macrophage phenotype. (B) Volcano plot displaying differentially expressed genes (DEGs) between the control and IL-4-treated macrophages. (C) Heatmap demonstrating the expression of key macrophage polarization markers. (D) Multi-pathway Gene Set Enrichment Analysis (GSEA) demonstrating the integrated enrichment of indicated pathways regulating macrophage functions. (E) Schematic illustration of macrophage priming for efferocytosis mediated by IL-4 and Arg. (F) Relative cell viability of macrophages treated with different concentrations of Arg at 24, 48, and 72 h. (G and H) Macrophage proliferation evaluated by 5-ethynyl-2′-deoxyuridine (EdU) assay, showing representative fluorescence images of EdU and 4',6-diamidino-2-phenylindole (DAPI) staining at 24, 48, and 72 h (G) and quantification of EdU-positive cells treated with 2 mM Arg (H). (I) Schematic illustration of the Transwell migration assay. (J and K) Macrophage recruitment evaluated by Transwell assay, showing representative crystal violet staining images of migrated macrophages after 48 h (J) and quantification of the number of migrated macrophages (K). Error bars represent SD. Data were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test (F and H) or one-way ANOVA followed by Tukey’s post hoc test (K). Sample sizes: n = 3 biological replicates (B–D and F); n = 5 biological replicates, each performed with 3 technical replicates (G, H, J, and K). Scale bars, 25 μm (G), 500 μm (J, top), and 100 μm (J, bottom).

Article Snippet: Cell proliferation was observed by laser confocal microscope at 24 h, 48 h, 72 h. Briefly, the proliferation of cells was assessed by BeyoClick EdU-488 Cell Proliferation Kit (Beyotime) according to the manufacturer’s instructions.

Techniques: Migration, Control, Expressing, Analysis, EdU Assay, Fluorescence, Staining, Transwell Migration Assay, Transwell Assay

Journal: iScience

Article Title: Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models

doi: 10.1016/j.isci.2026.117673

Figure Lengend Snippet:

Article Snippet: Sections were then washed in 1 x PBS four times and incubated in Alexa Fluor 488 AffiniPure Donkey Anti-Mouse IgG (H + L, Jackson ImmunoResearch, cat no: 715–545-150, West Grove, Pennsylvania), 1:200 dilution in 1% normal donkey serum in 0.5% Triton X-100 overnight at 4°C.

Techniques: Recombinant, Mutagenesis, Software, Analysis, Silicon